TRIM21-mediated K63-linked ubiquitination of G3BP1
🧬 G3BP1
🏥 N/A
🔗 4 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Materials: Recombinant TRIM21 E3 ligase, purified G3BP1 protein, E1/E2 enzymes (UBE1, UbcH5c), K63-only ubiquitin chains...
Expected: Quantitative predictions: (1) TRIM21 co-IPs with G3BP1 (>5-fold enrichment over IgG control, p<0.01)...
SPP1-mediated microglial synaptic engulfment assay
🧬 SPP1
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Primary Cell Culture and SPP1 Manipulation** — Week 1-2
Expected: 1. Primary: SPP1 knockdown will reduce microglial synaptic engulfment by >40% compared to scrambled ...
cGAS/STING pathway activation by TDP-43-released mtDNA
🧬 CGAS
🏥 amyotrophic lateral sclerosis
🔗 1 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: iPSC-derived Motor Neuron Culture and TDP-43 Manipulation** — Week 1-3
Expected: 1. Primary: cGAS/STING inhibition will reduce TDP-43-induced NF-κB activation by >60% (p < 0.01, Coh...
GWAS meta-analysis of major depressive disorder across three cohorts
🧬 N/A
🏥 major depressive disorder
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Cohort Preparation and Quality Control** — Month 1-2
Expected: 1. Primary: Identify 15-25 genome-wide significant loci (p < 5×10^-8) associated with MDD risk with ...
QTJD effects on macrophage polarization and inflammatory response
🧬 CD86, CXCL10, CD206, ARG1, MRC1
🏥 Mycoplasma pneumoniae pneumonia
🔗 3 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### PHASE 1: Macrophage Isolation and Culture (Day 0-2)
Expected: **CD86 mRNA expression**: QTJD treatment will increase CD86 transcript levels by 2.8 ± 0.4-fold at 5...
Human Reference Interactome (HuRI) mapping
🧬 N/A
🏥 Mendelian diseases
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Human Protein Library Construction and Validation** — Month 1-3
Expected: 1. Primary: Generate >50,000 high-confidence binary protein-protein interactions representing 4-fold...
Double depletion rescue experiment: tau and MAP6 co-depletion
🧬 Mapt, MAP6
🏥 N/A
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Primary Neuronal Culture Preparation and Transfection** — Days 1-7
Expected: 1. Primary: Complete morphological rescue in tau+MAP6 co-depleted neurons, with dendritic complexity...
MSC to chondrocyte mitochondrial transfer quantification
🧬 N/A
🏥 osteoarthritis
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 1: Cell Culture & Preparation (Day 0)
Expected: **Baseline transfer rate at 24h**: 12-18% of chondrocytes will display double-positive mitochondrial...
Cx43 and GJA1-20k overexpression effects on mitochondrial transfer
🧬 GJA1
🏥 osteoarthritis
🔗 3 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 1: Cell Culture & Baseline Characterization (Days 1-7)
Expected: Cx43-overexpressing MSCs will show a **2.5-3.5× increase** in mitochondrial transfer to chondrocytes...
IDH1 and IDH2 mutation sequencing in CNS and non-CNS tumors
🧬 IDH1, IDH2
🏥 gliomas (astrocytomas, oligodendrogliomas, glioblastomas)
🔗 1 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**IDH1 and IDH2 Mutation Sequencing in CNS and Non-CNS Tumors**
Expected: **CNS tumor cohort**: IDH1/IDH2 mutation frequency of 75-85% in astrocytomas (WHO II-IV) and oligode...
DPYD polymorphisms association with fluoropyrimidine ADRs
🧬 DPYD
🏥 cancer (patients receiving fluoropyrimidine chemotherapy)
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Real-Time PCR analysis of DNA extracted from 3ml whole blood samples to genotype nine DPYD variants: c.496A>G, c.1236G>A...
Expected: identification of DPYD variants significantly associated with fluoropyrimidine ADRs that could be us...
Meta-analysis of LRP1 C766T polymorphism and Alzheimer's disease susceptibility
🧬 LRP1
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Systematic literature review and meta-analysis of case-control studies. Studies were identified and data extracted for g...
Expected: Clarification of the association between LRP1 C766T polymorphism and AD risk across different popula...
FLS Stimulation and Transcriptomic Analysis
🧬 N/A
🏥 rheumatoid arthritis
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 1: 细胞培养与准备工作（第1-3天）
Expected: **IL-6分泌水平**：carH3-免疫复合物高浓度组（10 μg/mL）在24小时时间点将比vehicle对照组增加8-12倍（预计范围：800-1500 pg/mL vs. 对照组80-120 ...
Single-cell RNA-seq analysis of mouse testes with gonadal defects
🧬 Mlh3, Hormad1, Cul4a, Cnp
🏥 male infertility
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### 单细胞RNA-seq分析小鼠睾丸基因缺陷与精子发生缺陷的基因调控程序和细胞群体研究
Expected: **细胞捕获效率与数据质量**
Circadian gene expression effects of SD vs ketamine
🧬 PER1,PER2,CRY1,CRY2,BMAL1,CLOCK
🏥 depression
🔗 3 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Study Design Overview
Expected: **Ketamine acutely upregulates PER1 and PER2** — In the KET group, PER1 expression in PFC at ZT6 wil...
GWAS of plasma pTau181 in East Asian cohort
🧬 KCNJ3, APOE
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
---
Expected: **APOE ε4 effect on pTau181**: APOE ε4 carriers will show plasma pTau181 levels 1.5-2.0 pg/mL higher...
GWAS of plasma NfL in East Asian cohort
🧬 KLHDC4, APOE
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### GWAS of Plasma Neurofilament Light Chain (NfL) in East Asian Cohort (K-ROAD)
Expected: **APOE ε4 dose-response:** Each ε4 allele associated with β=0.12 (95% CI: 0.08-0.16) increase in log...
GWAS of plasma GFAP in East Asian cohort
🧬 SLC10A7, APOE
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 0: Study Design & Population Recruitment (Weeks 1-8)
Expected: **Primary signal at APOE locus:** rs429358 associated with plasma GFAP at p = 1.2×10⁻¹², ε4 carriers...
GWAS of plasma pTau217 in East Asian cohort
🧬 DACT1, DAAM1, APOE
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 1: Cohort Recruitment & Baseline Assessment (Month 1-6)
Expected: **APOE ε4 dose-response with pTau217**: ε4/ε4 carriers will show 2.1× higher baseline plasma pTau217...
Nation-wide NGS-based genetic screening of LGMD patients in US
🧬 CAPN3, DYSF, FKRP, ANO5, DNAJB6
🏥 Limb-girdle muscular dystrophy (LGMD)
🔗 1 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
### Phase 1: Study Design & Recruitment (Months 1-6)
Expected: **Recruitment Yield**: 2,000 patients enrolled across 30+ sites within 18 months (mean 67 patients/s...
GPR109A receptor validation using knockout macrophages
🧬 GPR109A, MAPK1, MAPK14, MAPK8, MAPK9
🏥 Mycoplasma pneumoniae pneumonia
🔗 1 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Western blotting analysis of MAPK proteins in GPR109A knockout macrophages treated with QTJD
Expected: GPR109A-dependent effects on ERK1/2 but not p38/JNK1/2
Proximity proteomics of VE-cadherin interactome in lymphatic endothelial cells
🧬 CDH5
🏥 N/A
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
proximity proteomics analysis with adrenomedullin treatment to trigger junctional reorganization
Expected: identification of novel VE-cadherin interacting proteins and understanding of junctional remodeling ...
TRIM21 E3 ubiquitin ligase screen for stress granule regulation
🧬 TRIM21
🏥 neurodegenerative diseases
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Screen design: Test 6 E3 ligases enriched in SG proteomics: TRIM21, TRIM32, STUB1, HUWE1, UBR4, MARCH5. Cells: U2OS stab...
Expected: Quantitative predictions: (1) Screen: TRIM21 knockdown increases SG count 2-3 fold and SG size 1.5-2...
Autophagy receptor identification for stress granule elimination
🧬 SQSTM1, CALCOCO2
🏥 neurodegenerative diseases
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Screen design: Test 8 canonical autophagy receptors: SQSTM1/p62, CALCOCO2/NDP52, NBR1, OPTN, TAX1BP1, TOLLIP, BNIP3, FUN...
Expected: Quantitative predictions: (1) Screen: SQSTM1 and CALCOCO2 show >70% colocalization with G3BP1 at SG ...
G3BP1-mediated stress granule assembly via liquid-liquid phase separation
🧬 G3BP1
🏥 N/A
🔗 4 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Constructs: G3BP1 full-length, ΔAcidic (deletion of IDR1, aa 1-139), ΔNTR (deletion of IDR2, aa 1-231), ΔC-term (deletio...
Expected: Quantitative predictions: (1) Full-length G3BP1 forms droplets at >5 μM protein + 10 ng/μL RNA. ΔAci...
VSMC cholesterol efflux and P2RY12 signaling
🧬 P2RY12
🏥 atherosclerosis
🔗 2 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Cells: Primary human coronary artery SMCs (ScienCell) or rat A7r5 SMCs, passages 3-6. Culture in SMCM medium with 5% FBS...
Expected: Quantitative predictions: (1) Baseline NBD-cholesterol efflux: 15-20% at 4h with apoA-I. (2) 2-MeSAD...
P2RY12 regulation of autophagy in VSMCs
🧬 P2RY12, ATG5
🏥 atherosclerosis
🔗 2 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
Cells: Primary human aortic SMCs (HASMCs, Lonza) passages 4-7, cultured in SmGM-2 medium. Cholesterol loading: 50 μg/mL ...
Expected: Quantitative predictions: (1) 2-MeSADP treatment: LC3-II/I ratio decreases to 0.3-0.4 (baseline ~0.6...
SPP1 upregulation in perivascular cells in AD mouse models
🧬 SPP1
🏥 Alzheimer's disease
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Animal Model Preparation and Tissue Collection** — Weeks 1-2
Expected: 1. Primary: >3-fold increase in perivascular macrophage SPP1 expression in 5xFAD mice compared to co...
Single-cell RNA sequencing analysis of human atherosclerotic plaques
🧬 C1Q (C1QA, C1QB, C1QC)
🏥 Atherosclerosis
🔗 3 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Dataset Acquisition and Quality Control** — Days 1-3
Expected: 1. Primary: Successful identification of 24 distinct cell clusters consolidating into 12 major cell ...
Machine learning-based identification of C1Q hub genes
🧬 C1QA, C1QC
🏥 Atherosclerosis
🔗 5 hyp
💰 N/A
⏱ N/A
✅ 0.00
✨ 0.00
**Phase 1: Dataset Preparation and Feature Selection** — Days 1-4
Expected: 1. Primary: C1QA and C1QC identified as top 2 hub genes with combined importance scores >0.8 across ...