🧪 Experiments

202 proposed experiments from the Forge — designed to validate, falsify, and explore hypotheses

202
Total Experiments
0.73
Avg Score
0.50
Avg Feasibility
161
Clinical
202
Validation
27
Falsification
All Types clinical (161)exploratory (242)falsification (27)validation (202)
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validation 0.95
Sevoflurane-induced neurotoxicity (SIN) rat model
🧬 C1qa 🏥 perioperative neurocognitive disorders 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Animal Preparation and Anesthesia Protocol** — Week 1
Expected: 1. Primary: Prolonged sevoflurane will increase MWM escape latency by >50% and reduce probe trial ti...
validation 0.95
Bmal1 knockout in mPFC excitatory neurons
🧬 BMAL1,HOMER1A,CAMK2A 🏥 depression 🔗 2 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### PHASE 1: Pre-Surgical Preparation & Baseline Assessment (Weeks 1–2)
Expected: **Conditional Bmal1 knockout efficiency**: Cre-mediated excision will reduce Bmal1 mRNA by **78–85%*...
validation 0.95
Chi3l1 deletion in APP/PS1 mouse model of Alzheimer's disease
🧬 Chi3l1 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Study Design
Expected: **Amyloid plaque burden (Thioflavin-S)** in APP/PS1;Chi3l1^-/- will be 35–45% lower than APP/PS1;Chi...
validation 0.95
Anti-ASC antibody intracerebroventricular injection in APP/PS1 mice
🧬 ASC 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Intracerebroventricular administration of anti-ASC N-terminal or C-terminal antibodies to APP/PS1 mice, followed by cogn...
Expected: Improved cognitive function and reduced AD pathology following ASC antibody treatment
validation 0.95
Myricanol treatment in HFD-induced hyperlipidemic C57BL/6J mice
🧬 ACSL1, SCD1, PPARγ 🏥 hyperlipidemia
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
8-week HFD induction followed by 25-day treatment with myricanol (100 or 150 mg/kg) or rosiglitazone (0.78 mg/kg). Multi...
Expected: Reduction in serum lipid levels, decreased hepatic lipid accumulation, weight loss, and modulation o...
validation 0.95
PS-NMP shape-dependent colonic barrier damage in mice
🧬 N/A 🏥 intestinal barrier dysfunction
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
120 five-week-old male C57BL/6J mice divided into seven groups and orally exposed to different doses of PS-NMPs (beads a...
Expected: Different shaped PS-NMPs would cause varying degrees of intestinal barrier damage with distinct mole...
validation 0.95
Curcumin-Licorice Combination in D-galactose/Sodium Nitrite AD Model
🧬 N/A 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Mice received D-galactose/sodium nitrite to induce AD pathology, then treated with curcumin (100 mg/kg), G. glabra (100 ...
Expected: Synergistic improvement in cognitive function and reduction in neuroinflammation with combination th...
validation 0.95
Electroacupuncture ST37 treatment for DSS-induced colitis in mice
🧬 CHAT, CHRNA7 🏥 colitis 🔗 3 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Mice received 2.5% DSS solution for 7 days to induce colitis, followed by electroacupuncture treatment at ST37 acupoint....
Expected: EA treatment was expected to and did reduce colitis severity, decrease pro-inflammatory cytokines, a...
validation 0.95
AECII-specific HMGCS2 overexpression in mouse pulmonary fibrosis
🧬 HMGCS2 🏥 idiopathic pulmonary fibrosis 🔗 4 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Phase 1: Animal Model Preparation & Gene Delivery (Days 0-14)
Expected: **HMGCS2 overexpression validation**: AAV-HMGCS2 group will show ≥3.2-fold increase in lung HMGCS2 m...
validation 0.95
ABT263 treatment in sublethally irradiated mice
🧬 N/A 🏥 radiation-induced premature aging
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Study Design Overview
Expected: **Survival**: 100% survival in sham groups; ≥85% survival in TBI groups (ABT263 may slightly reduce ...
validation 0.95
Long-term perampanel treatment in A53T BAC-SNCA mice with PFF injection
🧬 SNCA 🏥 Lewy body disease 🔗 1 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Study Overview
Expected: **Motor deficit onset:** Vehicle-treated A53T/PFF mice will show significant rotarod impairment by 3...
validation 0.95
ABT263 treatment in naturally aged mice
🧬 N/A 🏥 natural aging
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
### Study Design
Expected: **Senescent cell clearance**: ABT263 will reduce SA-β-gal+ cells by 40-60% in liver, lung, and adipo...
validation 0.90
CD2AP downregulation in brain endothelial cells - memory function
🧬 CD2AP 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
genetic downregulation of CD2AP in brain vascular endothelial cells, behavioral testing
Expected: memory impairment in mice with reduced endothelial CD2AP
validation 0.90
Cerebrovascular function analysis in CD2AP mutant mice
🧬 CD2AP 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
measurement of blood flow regulation, neurovascular coupling, mural cell activity assessment
Expected: altered blood flow regulation and mural cell defects
validation 0.90
Reelin glycoprotein treatment rescue experiment
🧬 CD2AP 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
reelin glycoprotein treatment, measurement of cerebral blood flow, amyloid-β challenge
Expected: increased cerebral blood flow and protection against amyloid-β in male mice
validation 0.90
P2RY12 inhibition in atherosclerosis mouse model
🧬 P2RY12 🏥 atherosclerosis 🔗 2 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Animals: ApoE-/- mice (C57BL/6 background), 8-10 weeks old, n=15 per group (vehicle, clopidogrel 10mg/kg/day, ticagrelor...
Expected: Quantitative predictions: (1) Vehicle HFD group: aortic root plaque area 400,000-500,000 μm², 60-70%...
validation 0.90
Endothelial CD2AP knockdown effects on memory in male mice
🧬 CD2AP 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
genetic downregulation of CD2AP in brain endothelial cells followed by behavioral memory testing
Expected: reduced endothelial CD2AP impairs memory function in male mice
validation 0.90
Spp1 knockout prevents synaptic loss in AD mouse models
🧬 Spp1 🏥 Alzheimer's disease 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
**Phase 1: Generation of Spp1 Knockout AD Mouse Models** — Weeks 1-4
Expected: 1. Primary: Spp1-/-;5xFAD mice show 60-80% preservation of hippocampal synaptic density compared to ...
validation 0.90
Sevoflurane-induced neurotoxicity (SIN) model in rats
🧬 C1qa 🏥 perioperative neurocognitive disorders (PND) 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Prolonged sevoflurane anesthesia exposure followed by behavioral testing (Morris water maze, elevated plus maze, open fi...
Expected: Prolonged anesthesia would cause neuroinflammation and complement-mediated synaptic elimination lead...
validation 0.90
Microglial depletion rescue experiment
🧬 N/A 🏥 perioperative neurocognitive disorders
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Microglial depletion followed by prolonged sevoflurane anesthesia and assessment of neuroinflammation, complement activa...
Expected: Prevention of neuroinflammation, complement activation, synaptic loss, and behavioral deficits
validation 0.90
C1q neutralization experiment
🧬 C1qa 🏥 perioperative neurocognitive disorders 🔗 5 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
C1q neutralization followed by prolonged sevoflurane anesthesia and assessment of complement activation and synaptic int...
Expected: Reduced complement activation and preserved synaptic integrity
validation 0.90
TDP-43 mutant mouse model cGAS/STING pathway analysis
🧬 TARDBP 🏥 amyotrophic lateral sclerosis 🔗 4 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Analysis of TDP-43 mutant mice with pharmacologic inhibition or genetic deletion of cGAS/STING components, assessment of...
Expected: cGAS/STING inhibition reduces neuroinflammation in TDP-43 mutant mice
validation 0.90
Continental-scale satellite tracking of Arctic peregrine falcons
🧬 N/A 🏥 N/A
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
satellite GPS tracking of 56 individuals from 6 Arctic breeding populations throughout annual migration cycles
Expected: identification of distinct migration routes and population-specific migration patterns
validation 0.90
Continental-scale satellite tracking of peregrine falcon migration
🧬 N/A 🏥 N/A
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
satellite tracking of 56 peregrine falcons from 6 Arctic breeding populations across Eurasia
Expected: identification of distinct migration routes and understanding of route formation and maintenance
validation 0.90
Muscle-specific Tug knockout mice analysis
🧬 Aspscr1/TUG 🏥 diabetes/metabolic dysfunction
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Muscle-specific gene knockout using Cre-lox system, metabolic phenotyping, glucose uptake measurements
Expected: Impaired glucose uptake and altered energy expenditure due to loss of TUG-mediated GLUT4 regulation
validation 0.90
Constitutive TUG cleavage mouse model
🧬 Aspscr1/TUG 🏥 metabolic regulation
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Transgenic mouse generation, gene expression analysis, metabolic measurements, nuclear localization studies
Expected: Enhanced expression of thermogenic genes like sarcolipin and UCP1, increased energy expenditure
validation 0.90
PTEC-specific RUBCN knockout mice phenotype analysis
🧬 RUBCN 🏥 kidney injury/metabolic syndrome
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Generation of tissue-specific knockout mice, histological analysis of kidney tissue, metabolic profiling, assessment of ...
Expected: Protection from kidney injury due to enhanced autophagy
validation 0.90
PGC-1α knockout effects on PV+ interneuron maturation
🧬 PPARGC1A 🏥 N/A 🔗 2 hyp
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
# PGC-1α Knockout Effects on PV+ Interneuron Maturation Protocol
Expected: ## Primary Outcomes
validation 0.90
Muscle-specific Tug knockout mice study
🧬 Tug (Aspscr1) 🏥 Diabetes/metabolic dysfunction
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Generation of muscle-specific knockout mice, metabolic phenotyping, glucose uptake assays, gene expression analysis
Expected: Reduced glucose uptake and altered energy expenditure due to loss of TUG cleavage pathway
validation 0.90
Muscle-specific constitutive TUG cleavage mice study
🧬 Tug (Aspscr1) 🏥 Metabolic regulation
💰 N/A ⏱ N/A ✅ 0.00 ✨ 0.00
Generation of constitutive cleavage model, metabolic analysis, gene expression profiling, thermogenesis measurements
Expected: Enhanced thermogenesis and lipid oxidation due to continuous TUG C-terminal product activity
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