Validation experiment designed to validate causal mechanisms targeting N/A in C57BL/6 mice. Primary outcome: Spatial memory performance and neuroinflammatory markers
This comprehensive animal study evaluated the synergistic neuroprotective effects of curcumin and Glycyrrhiza glabra (licorice) in a chemically-induced Alzheimer's disease mouse model. Eighty C57BL/6 mice were divided into eight experimental groups to test various treatment conditions including monotherapies, combination therapies at different doses, and positive controls. The study employed D-galactose and sodium nitrite to induce AD-like pathology and neuroinflammation. Cognitive function was assessed using Morris Water Maze testing, while molecular analyses examined tau-related proteins, inflammatory pathways, oxidative stress markers, and apoptotic factors. The research aimed to demonstrate that the combination therapy could simultaneously target upstream (TLR4/MyD88) and downstream (NF-κB) components of neuroinflammation through vertical cooperation between the two compounds.
Mice received D-galactose/sodium nitrite to induce AD pathology, then treated with curcumin (100 mg/kg), G. glabra (100 mg/kg), low-dose combination (50+50 mg/kg), high-dose combination (100+100 mg/kg), donepezil (3 mg/kg), or SN50 (400 µg/kg). Cognitive testing via Morris Water Maze and molecular analyses of tau proteins, inflammatory mediators, and oxidative stress markers.
Synergistic improvement in cognitive function and reduction in neuroinflammation with combination therapy compared to monotherapies
Significant reduction in escape latency, decreased inflammatory markers (IL-6, TNF-α), reduced tau pathology, and improved antioxidant defense
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