Blood metabolite analysis of purinergic molecules in COVID-19

Clinical Score: 0.800 Price: $0.50 COVID-19 human peripheral blood from COVID-19 patients Status: proposed

What This Experiment Tests

Clinical experiment designed to assess clinical efficacy targeting N/A in human peripheral blood from COVID-19 patients. Primary outcome: levels of purinergic metabolites and correlation with coagulation proteins

Description

Proteomic analysis of blood samples from COVID-19 patients to measure levels of purinergic signaling molecules including ATP, ADP, AMP, inosine, and hypoxanthine using mass spectrometry. The study examined correlations between these nucleotides/nucleosides and coagulation proteins (fibrinogen and other coagulation factors) as well as clinical features. Blood metabolite levels were compared between COVID-19 patients at different disease stages and healthy controls or non-COVID-19 patients. The analysis revealed stage-dependent changes in metabolite levels and significant correlations with coagulation markers.

TARGET GENE
N/A
MODEL SYSTEM
human peripheral blood from COVID-19 patients
ESTIMATED COST
$0
TIMELINE
0 months
PATHWAY
purinergic signaling pathway, coagulation pathway
SOURCE
extracted_from_pmid_35091759
PRIMARY OUTCOME
levels of purinergic metabolites and correlation with coagulation proteins

Scoring Dimensions

Info Gain 0.00 (25%) Feasibility 0.00 (20%) Hyp Coverage 0.00 (20%) Cost Effect. 0.00 (15%) Novelty 0.00 (10%) Ethical Safety 0.00 (10%) 0.800 composite

📖 Wiki Pages

ATP P2X3 Receptor NeuronscellAMP-PD: Accelerating Medicines Partnership: ParkindatasetCOVID-19 Neurodegeneration Mechanismmechanism

Protocol

Raw proteomics data acquired using mass spectrometry from public datasets (GEO), analyzed for ATP, ADP, AMP, inosine, hypoxanthine levels and correlations with fibrinogen and coagulation proteins

Expected Outcomes

Altered purinergic metabolite levels in COVID-19 patients with correlations to coagulation markers

Success Criteria

Significant changes in metabolite levels and correlations with coagulation proteins

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