ASC antibody treatment in SH-SY5Y-APP695 cells

This experiment investigated the effects of ASC neutralizing antibodies targeting different domains of the ASC protein on cell apoptosis and amyloid-β (Aβ) production in vitro. SH-SY5Y-APP695 cells, which are human neuroblastoma cells transfected to overexpress APP695, were treated with either anti-ASC N-terminal antibodies (targeting the pyrin domain) or anti-ASC C-terminal antibodies (targeting the caspase recruitment domain). The study measured cell apoptosis, mitochondrial damage, and Aβ production levels to assess the differential roles of the two ASC domains in Alzheimer's disease pathogenesis. The mechanism of action was explored by examining BACE1 inhibition, as BACE1 is a key enzyme in Aβ production. Results showed that both antibody treatments reduced apoptosis, mitochondrial damage, and Aβ production through BACE1 inhibition.