Exploratory experiment designed to discover new patterns targeting SNCA in mouse primary hippocampal neurons. Primary outcome: neuronal uptake of PFFs
This experiment examined the mechanisms of neuronal uptake of α-synuclein preformed fibrils (PFFs) in primary hippocampal neurons. PFFs were labeled with pH-sensitive dye to track uptake. Neurons were treated with either dynamin inhibitors or clathrin inhibitors to determine which endocytic pathway is responsible for PFF uptake. The study aimed to understand whether PFF uptake occurs through clathrin-mediated endocytosis or dynamin-dependent mechanisms.
PFFs labeled with pH-sensitive dye were applied to cultured neurons, with treatment using dynamin or clathrin inhibitors
PFF uptake would be blocked by dynamin inhibitors but not clathrin inhibitors
significant reduction in PFF uptake with dynamin inhibition
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