Reelin glycoprotein treatment rescue experiment

Validation Score: 0.900 Price: $0.50 Alzheimer's disease mice Status: proposed

What This Experiment Tests

Validation experiment designed to validate causal mechanisms targeting CD2AP in mice. Primary outcome: restoration of cerebral blood flow and protection against Aβ toxicity

Description

Treatment of CD2AP mutant mice with reelin glycoprotein to test whether this intervention could mitigate the effects of CD2AP loss of function via ApoER2 signaling. The experiment examined whether reelin treatment could restore cerebrovascular function and protect against amyloid-β toxicity. Results showed increased resting cerebral blood flow and protection of male mice against amyloid-β effects, demonstrating the therapeutic potential of reelin in addressing CD2AP-related vascular dysfunction.

TARGET GENE
CD2AP
MODEL SYSTEM
mice
ESTIMATED COST
$0
TIMELINE
0 months
PATHWAY
reelin-ApoER2 signaling
SOURCE
extracted_from_pmid_39892386
PRIMARY OUTCOME
restoration of cerebral blood flow and protection against Aβ toxicity

Scoring Dimensions

Info Gain 0.00 (25%) Feasibility 0.00 (20%) Hyp Coverage 0.00 (20%) Cost Effect. 0.00 (15%) Novelty 0.00 (10%) Ethical Safety 0.00 (10%) 0.900 composite

📖 Wiki Pages

CD2AP Gene — CD2-Associated ProteingeneCD2AP Synaptic Dysfunction Alzheimer's Disease CaumechanismCD2AP ProteinproteinAlzheimer's Disease Genetic VariantsdiseaseAlzheimer's Disease vs Parkinson's Disease ComparidiseaseAPP Mutations in Alzheimer's DiseasediseaseDLB, Parkinson's Disease, and Alzheimer's Disease:diseaseEarly-Onset Alzheimer's Disease (EOAD)diseaseInvestment Landscape: Alzheimer's DiseasediseasePreclinical Alzheimer's DiseasediseaseProdromal Alzheimer's DiseasediseasePSEN1 Mutations in Alzheimer's DiseasediseasePSEN2 Mutations in Alzheimer's DiseasediseaseSporadic vs Familial Alzheimer's Disease: ComprehediseaseTREM2 Variants in Alzheimer's Diseasedisease

Protocol

reelin glycoprotein treatment, measurement of cerebral blood flow, amyloid-β challenge

Expected Outcomes

increased cerebral blood flow and protection against amyloid-β in male mice

Success Criteria

significant improvement in blood flow and reduced amyloid-β toxicity

Related Hypotheses (5)

APOE4-Specific Proteolytic Fragment Inhibition Therapy0.777
TREM2-mediated microglial tau clearance enhancement0.594
LRP1-Dependent Tau Uptake Disruption0.576
VCP-Mediated Autophagy Enhancement0.571
Extracellular Vesicle Biogenesis Modulation0.558

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