Validation experiment designed to validate causal mechanisms targeting CERS2 in IL-10/CERS2 double knockout mice. Primary outcome: Inflammatory gene expression levels and disease severity
Genetic deletion study of ceramide synthase 2 (Cers2) to test whether removing the enzyme responsible for VLC ceramide production could limit the inflammatory gene expression program associated with IL-10 deficiency. This experiment involved creating double knockout mice lacking both IL-10 and CERS2 to determine if blocking VLC ceramide synthesis could rescue the inflammatory phenotype.
Generation of Cers2 knockout mice on IL-10 deficient background, assessment of inflammatory gene expression both in vitro and in vivo
CERS2 deletion limits exacerbated inflammatory gene expression in IL-10 deficient mice
Reduced inflammatory gene expression and improved disease phenotype in double knockout mice
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