SASP Factor Secretion Patterns and Their Impact on Neuronal Health

In-Vitro Score: 0.850 Price: $0.50 Alzheimer disease Primary astrocytes from aged donor tissue + primary cortical neurons Status: proposed

What This Experiment Tests

In-Vitro experiment designed to assess clinical efficacy targeting N/A in Primary astrocytes from aged donor tissue + primary cortical neurons. Primary outcome: Neuronal viability and SASP cytokine panel in co-culture

Description

Characterize the secretome (SASP) profile of senescent astrocytes and fibroblasts from aged brain tissue, and test whether pharmacological SASP modulation (e.g., rapamycin, navitoclax) preserves neuronal health in co-culture.

TARGET GENE

N/A

MODEL SYSTEM

Primary astrocytes from aged donor tissue + primary cortical neurons

ESTIMATED COST

$32,000

TIMELINE

7 months

PATHWAY

N/A

SOURCE

auto-generated

PRIMARY OUTCOME

Neuronal viability and SASP cytokine panel in co-culture

Scoring Dimensions

Protocol

in-vitro

Expected Outcomes

SASP-modulated co-cultures show >40% improvement in neuronal survival under stress conditions vs. untreated senescent co-cultures.

Success Criteria

Multiplex cytokine array (IL-6, IL-8, TNF-α, etc.), neuronal viability (Live/Dead assay); threshold: p < 0.05 for survival outcome.

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