Exploratory experiment designed to discover new patterns targeting multiple autophagy genes in NSC34 neuroblastoma x spinal cord cells. Primary outcome: mRNA expression levels of autophagy and lysosomal genes
This experiment examined the transcriptional changes induced by trehalose treatment in cellular models. Using RT-qPCR, the researchers measured expression levels of TFEB target genes and autophagy-related genes including Ppargc1a, lysosomal hydrolases (Ctsb, Gla, Tpp1), lysosomal membrane proteins (Lamp2a, Mcoln1), and autophagy components (Becn1, Atg10, Atg12, Sqstm1/p62, Map1lc3b, Hspb8, Bag3). The study demonstrated that most of these changes were dependent on PPP3 and TFEB activity.
RT-qPCR analysis of gene expression following trehalose treatment, with and without PPP3 inhibition or TFEB silencing
Upregulation of TFEB target genes and autophagy-related genes in a PPP3- and TFEB-dependent manner
Significant upregulation of target genes that is blocked by PPP3 inhibition or TFEB silencing
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