Exploratory experiment designed to discover new patterns targeting N/A in C57BL/6 mice, human AECIIs, human lung fibroblasts. Primary outcome: lipid accumulation patterns
Oil-red staining and immunofluorescence analysis were performed to detect lipid accumulation in mouse lung fibrosis frozen sections and bleomycin-treated human type II alveolar epithelial cells (AECIIs) and lung fibroblasts. This histological analysis aimed to identify the cellular sources and distribution patterns of abnormal lipid deposits in pulmonary fibrosis models, establishing that injured AECIIs are the primary source of accumulated lipids in response to bleomycin stimulation.
Oil-red staining and immunofluorescence microscopy on frozen tissue sections and cultured cells treated with bleomycin
Identification of cellular sources of lipid accumulation in pulmonary fibrosis
Detection of lipid deposits in specific cell types
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