Exploratory experiment designed to discover new patterns targeting multiple autophagy genes in neuronal cell lines. Primary outcome: mRNA expression levels of autophagy genes
This experiment used RT-qPCR to analyze the expression of multiple autophagy-related genes following trehalose treatment. The study examined TFEB target genes including Ppargc1a, lysosomal hydrolases and membrane proteins (Ctsb, Gla, Lamp2a, Mcoln1, Tpp1), and autophagy-related components (Becn1, Atg10, Atg12, Sqstm1/p62, Map1lc3b, Hspb8, and Bag3). The researchers demonstrated that most of these genes were upregulated in a PPP3- and TFEB-dependent manner, confirming the transcriptional regulation of autophagy by trehalose through the TFEB pathway.
RNA extraction, reverse transcription, real-time quantitative PCR analysis of multiple autophagy-related genes
Significant upregulation of TFEB target genes and autophagy-related genes following trehalose treatment
Statistically significant increase in mRNA levels of autophagy genes that is dependent on PPP3 and TFEB
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