Validation experiment designed to validate causal mechanisms targeting NPM1 in syngeneic mice with subcutaneous tumor xenografts. Primary outcome: tumor progression and survival rates
Subcutaneous inoculation of Npm1-deficient tumor cells into syngeneic mice to examine the roles of NPM1 in tumor progression and tumor microenvironment reprogramming. The study evaluated tumor growth kinetics, survival of tumor-bearing mice, immune cell infiltration patterns, and activation states using comprehensive flow cytometry, CyTOF, immunohistochemistry staining, and RNA-seq analysis. Loss of NPM1 was shown to inhibit tumor progression and enhance survival, with increased CD8+ T cell infiltration and activation alongside reduced presence of immunosuppressive cells.
Subcutaneous inoculation of Npm1-deficient tumor cells followed by monitoring tumor growth, survival analysis, and comprehensive immune profiling using CyTOF, flow cytometry, immunohistochemistry, and RNA-seq
NPM1 deficiency would affect tumor progression and immune microenvironment
Significant changes in tumor growth, survival, and immune cell populations
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