In-Vitro experiment designed to assess clinical efficacy targeting N/A in iPSC-derived microglia-astrocyte-neuron triple co-culture. Primary outcome: Astrocyte neuroprotective marker panel and neuron survival under oxidative stress
Elucidate the molecular mechanisms by which TREM2 expressed on microglia mediates beneficial cross-talk with astrocytes, and how this axis is disrupted in TREM2 deficiency models of neurodegeneration.
in-vitro
TREM2 knockdown in microglia reduces astrocyte activation toward neuroprotective phenotype (reduced GFAP, increased S100A10), and co-culture supernatant is less protective against oxidative stress injury.
Astrocyte phenotype markers (RT-qPCR, ELISA: GFAP, S100A10, IL-10); neuron survival after oxidative stress (MTT/WST-8); threshold: p < 0.05.
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