Validation experiment designed to validate causal mechanisms targeting TLR2, SNCA in Thy1.2-α-synuclein transgenic mice with TLR2 reporter construct. Primary outcome: TLR2 promoter activity over time
A longitudinal bioluminescence imaging study was conducted using Thy1.2-α-synuclein transgenic mice that expressed a bicistronic DNA construct containing reporter genes (luciferase and green fluorescent protein) under the transcriptional control of the murine toll-like receptor 2 promoter. Monthly imaging revealed progressive increases in TLR2 expression starting from 10 months of age. This experiment provided real-time monitoring of TLR2 activation over time in living animals with α-synuclein pathology, demonstrating that α-synuclein overexpression leads to a progressive microglial response and TLR2 upregulation with age-dependent onset.
Monthly bioluminescence imaging using luciferase reporter under TLR2 promoter control
Progressive increase in TLR2 expression with age
Detectable bioluminescence signal increase over time
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