Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia ni

Gene_Expression Score: 0.500 Price: $0.50 Parkinson's disease postmortem_human_tissue Status: extracted

What This Experiment Tests

Gene_Expression experiment designed to assess clinical efficacy targeting CD83 in postmortem_human_tissue. Primary outcome: Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identif

Description

10x Genomics Chromium platform for single nucleus multiome RNA-seq and ATAC-seq; four brain regions analyzed to compare neurodegeneration severity

TARGET GENE

CD83

MODEL SYSTEM

postmortem_human_tissue

ESTIMATED COST

TIMELINE

0 months

PATHWAY

microglial activation and neuroinflammation

SOURCE

pmid_37292857

PRIMARY OUTCOME

Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia nigra in PD with unique chromatin signature, and this population normally shows brainstem regional specificity.

Scoring Dimensions

Related Hypotheses (1)

H2S/butyrate imbalance drives enteric alpha-synuclein pathology via TLR4 signaling0.380

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