Enteric Alpha-Synuclein Propagation and ENS Dysfunction in PD Mouse Model

In-Vivo Score: 0.850 Price: $0.50 Parkinson disease Mouse (C57BL/6 or Thy1-αSyn transgenic), gut injection model Status: proposed

What This Experiment Tests

In-Vivo experiment designed to assess clinical efficacy targeting N/A in Mouse (C57BL/6 or Thy1-αSyn transgenic), gut injection model. Primary outcome: p-Syn (Ser129) pathology burden in vagus nerve and brainstem DMV

Description

Determine whether alpha-synuclein fibrils injected into the gut lumen propagate via the vagus nerve to the brainstem and whether ENS dysfunction constitutes a self-reinforcing feedback loop in a preformed PD mouse model.

TARGET GENE

N/A

MODEL SYSTEM

Mouse (C57BL/6 or Thy1-αSyn transgenic), gut injection model

ESTIMATED COST

$48,000

TIMELINE

12 months

PATHWAY

N/A

SOURCE

auto-generated

PRIMARY OUTCOME

p-Syn (Ser129) pathology burden in vagus nerve and brainstem DMV

Scoring Dimensions

Protocol

in-vivo

Expected Outcomes

Alpha-synuclein pathology detected in vagus nerve and dorsal motor nucleus by 6 months post-injection, with progressive ENS dysfunction evidenced by delayed gut transit.

Success Criteria

p-Syn (Ser129) IHC in vagus and DMV; threshold: pathology score > 2 in treatment vs. control groups.

Related Hypotheses (0)

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