Exploratory experiment designed to discover new patterns targeting N/A in human neutrophils. Primary outcome: histone carbamylation levels during NETosis
Neutrophil extracellular traps (NETs) were generated using calcium ionophore treatment and analyzed for carbamylation modifications. The study examined histone carbamylation, particularly of histone H3, during NETosis using multiple detection methods including immunofluorescence microscopy, flow cytometry, and mass spectrometry. The experiment aimed to characterize the extent and specificity of protein carbamylation that occurs during NET formation, with particular focus on identifying carbamylated histone H3 (carH3) as a major carbamylated autoantigen produced during this process.
calcium ionophore stimulation, immunofluorescence, flow cytometry, mass spectrometry
carbamylation of histones, particularly H3, during NET formation
detection of carbamylated proteins in NETs
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