Exploratory experiment designed to discover new patterns targeting NPM1, IRF1 in cultured cells. Primary outcome: protein-protein interaction between NPM1 and IRF1
Co-immunoprecipitation experiments to verify the physical interaction between NPM1 and the transcription factor IRF1. This experiment aimed to establish the molecular mechanism by which NPM1 regulates antigen presentation by demonstrating direct protein-protein interaction with IRF1, a key transcription factor involved in MHC gene expression regulation.
Co-immunoprecipitation assays to detect physical association between NPM1 and IRF1 proteins
Direct physical interaction between NPM1 and IRF1 proteins
Successful co-precipitation of NPM1 and IRF1
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