Validation experiment designed to validate causal mechanisms targeting CHRNA7 in Chrna7 knockout mice. Primary outcome: enhanced memory impairment on Novel Object Recognition test
Chrna7 knockout mice (Chrna7-/-) lacking functional α7 nicotinic acetylcholine receptors were fed a high-fat diet to determine the essential role of α7nAChR in protecting against diet-induced cognitive dysfunction. This genetic loss-of-function experiment tested whether absence of α7nAChR would exacerbate the negative cognitive effects of HFD exposure. The experiment provided mechanistic insight into the protective role of cholinergic signaling against neuroinflammation and memory impairment by comparing knockout mice to wild-type controls under HFD conditions.
HFD feeding in Chrna7-/- mice followed by behavioral testing and comparison to wild-type HFD-fed mice
Chrna7-/- mice would show greater cognitive impairment under HFD conditions compared to wild-type mice
Significantly worse performance in knockout mice compared to wild-type controls on HFD
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