Validation experiment designed to validate causal mechanisms targeting N/A in adult and aged mice. Primary outcome: microglial gene expression profile and inflammatory status
This comprehensive study examined the effects of dietary fiber supplementation on gut microbiome composition, short-chain fatty acid production, intestinal inflammation, and microglial gene expression in adult and aged mice. Mice were fed either a low fiber diet (1% cellulose) or high fiber diet (5% inulin) for 4 weeks. The study measured gut microbiome alterations, SCFA production (butyrate, acetate, and total SCFAs), histological inflammation scoring in the distal colon, and gene expression analysis of inflammatory markers, epigenetic regulators, and microglial sensory apparatus (sensome) in the brain. Results showed that high fiber diet altered gut microbiome, increased SCFA production, reduced colonic inflammation in aged animals, and promoted a more anti-inflammatory microglial profile in aged mice.
4-week dietary intervention with either 1% cellulose (low fiber) or 5% inulin (high fiber) diet, followed by gut microbiome analysis, SCFA measurement, histological assessment of colon inflammation, and brain gene expression analysis
High fiber diet would increase beneficial gut bacteria, increase SCFA production, reduce intestinal inflammation, and promote anti-inflammatory microglial phenotype
Significant changes in gut microbiome composition, increased SCFA levels, reduced colonic inflammation scores, and altered microglial gene expression toward anti-inflammatory profile
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