Delineation of the TRAK binding regions of the kinesin-1 motor proteins.

1. FEBS Lett. 2013 Nov 29;587(23):3763-9. doi: 10.1016/j.febslet.2013.09.049. Epub 2013 Oct 22. Delineation of the TRAK binding regions of the kinesin-1 motor proteins. Randall TS(1), Moores C, Stephenson FA. Author information: (1)University College London School of Pharmacy, 29/39 Brunswick Square, London WC1N 1AX, United Kingdom. Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 cargo domain, rationally designed C-terminal truncations of KIF5A and KIF5C were generated and their co-association with TRAK1/2 determined by quantitative co-immunoprecipitations following co-expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A. Copyright © 2013 Federation of European Biochemical Societies. All rights reserved. DOI: 10.1016/j.febslet.2013.09.049 PMCID: PMC3853714 PMID: 24161670 [Indexed for MEDLINE]