Hypothesis Comparison

graph TD
    A["LAMP1 Gene Expression"]
    B["LAMP1 Protein Synthesis"]
    C["Dynein Motor Complex"]
    D["Hook Adaptor Proteins"]
    E["Lysosome-Dynein Assembly"]
    F["Retrograde Lysosome Transport"]
    G["Perinuclear Lysosome Clustering"]
    H["Autophagosome-Lysosome Fusion"]
    I["Protein Aggregate Clearance"]
    J["Lysosome Dysfunction"]
    K["Impaired Autophagy"]
    L["Protein Accumulation"]
    M["Neuronal Cell Death"]
    N["Lysosome Positioning Modulators"]
    O["Motor Protein Enhancers"]

    A -->|"transcription and translation"| B
    B -->|"membrane insertion"| E
    C -->|"motor complex recruitment"| E
    D -->|"adaptor binding"| E
    E -->|"microtubule transport"| F
    F -->|"positioning regulation"| G
    G -->|"facilitates fusion"| H
    H -->|"degradation pathway"| I
    E -->|"dysfunction pathway"| J
    J -->|"impaired clearance"| K
    K -->|"accumulation cascade"| L
    L -->|"cellular toxicity"| M
    N -->|"therapeutic intervention"| E
    O -->|"motor enhancement"| C

    classDef mechanism fill:#4fc3f7
    classDef pathology fill:#ef5350
    classDef therapy fill:#81c784
    classDef outcome fill:#ffd54f
    classDef genetics fill:#ce93d8

    class A,B,C,D genetics
    class E,F,G,H,I mechanism
    class J,K,L,M pathology
    class N,O therapy

graph TD
    A["CYP46A1 Gene Therapy
Vector Delivery"] -->|"increases"| B["CYP46A1 Enzyme
Expression"]
    B -->|"converts"| C["Cholesterol to
24S-Hydroxycholesterol"]
    C -->|"crosses"| D["Blood-Brain Barrier
Efflux"]
    D -->|"reduces"| E["Brain Cholesterol
Levels"]
    E -->|"disrupts"| F["Lipid Raft
Microdomains"]
    F -->|"decreases"| G["gamma-Secretase
Activity"]
    G -->|"reduces"| H["Amyloid-beta
Production"]
    E -->|"modulates"| I["Cholesterol-dependent
APP Processing"]
    I -->|"shifts to"| J["Alpha-secretase
Pathway"]
    J -->|"increases"| K["sAPP-alpha
Neuroprotective Fragment"]
    H -->|"decreases"| L["Amyloid Plaque
Formation"]
    C -->|"activates"| M["LXR Nuclear
Receptors"]
    M -->|"upregulates"| N["ABCA1 and APOEpsilon
Expression"]
    N -->|"enhances"| O["Cholesterol and
Amyloid Clearance"]
    L -->|"reduces"| P["Neuroinflammation
and Tau Pathology"]
    K -->|"promotes"| Q["Synaptic Plasticity
and Neuronal Health"]
    O -->|"improves"| Q
    P -->|"prevents"| R["Cognitive Decline
and Neurodegeneration"]
    Q -->|"leads to"| R

    classDef normal fill:#4fc3f7,stroke:#2196f3
    classDef therapeutic fill:#81c784,stroke:#4caf50
    classDef pathology fill:#ef5350,stroke:#f44336
    classDef outcome fill:#ffd54f,stroke:#ff9800
    classDef molecular fill:#ce93d8,stroke:#9c27b0

    class A therapeutic
    class B,C,D,M,N molecular
    class E,F,G,I,J normal
    class H,L,P pathology
    class K,O,Q,R outcome